Bacteriophage T5 gene D10 encodes a branch-migration protein
نویسندگان
چکیده
منابع مشابه
Bacteriophage T5 gene D10 encodes a branch-migration protein
Helicases catalyze the unwinding of double-stranded nucleic acids where structure and phosphate backbone contacts, rather than nucleobase sequence, usually determines substrate specificity. We have expressed and purified a putative helicase encoded by the D10 gene of bacteriophage T5. Here we report that this hitherto uncharacterized protein possesses branch migration and DNA unwinding activity...
متن کاملRole of the T5 gene D15 nuclease in the generation of nicked bacteriophage T5 DNA.
The processing of newly replicated concatameric T5 DNA into both single stranded DNA changed of unit length and single-stranded fragments of sizes comparable to those found in mature T5 virion DNA occurs in the absence of late T5 protein synthesis. The formation of unit-length, single-stranded DNA chains does not require the early T5 gene D15 nuclease: however, the subsequent formation of the s...
متن کاملPolarized injection of the bacteriophage T5 chromosome.
Examination of the first-step-transfer DNA of T5ris mutants which carry new EcoRI sites showed that the left end of the chromosome is injected first.
متن کاملThe Arabidopsis STICHEL gene is a regulator of trichome branch number and encodes a novel protein.
Here, we analyze the STICHEL (STI) gene, which plays an important role in the regulation of branch number of the unicellular trichomes in Arabidopsis. We have isolated the STI locus by positional cloning and confirmed the identity by sequencing seven independent sti alleles. The STI gene encodes a protein of 1,218 amino acid residues containing a domain with sequence similarity to the ATP-bindi...
متن کاملGenetic suppressors of bacteriophage t5 amber mutants.
Nonsense mutations in structural genes appear to abort polypeptide chain synthesis at the amino acid site governed by the mutant codon. The two presently recognized types of nonsense mutant, amber and ochre, have been assigned the codons UAG and UAA, respectively. Certain bacteria harbor one or more genetic suppressors that counteract the effect of appropriate nonsense mutations in their own st...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scientific Reports
سال: 2016
ISSN: 2045-2322
DOI: 10.1038/srep39414